(Booth Talk from SC20)
Antibiotic-resistant bacteria is a challenge for public health. Pathogenic bacteria have resistance mechanisms that allow the bacteria to survive and grow when antibiotics are present. Specific genes code for resistance mechanisms and can spread to other bacteria of the same species and also across species through horizontal gene transfer. 27 clinical isolates from 2017 and 89 from 2020 have been collected from species from Enterobacter, Escherichia, Pseudomonas, Staphylococcus, Klebsiella, and Stenotrophomonas to identify antibiotic-resistance genes and resistance mechanisms. After sequencing, the raw-read files were assembled using SPAdes (3.12.0) and mobile genetic elements are being identified using plasmidSPAdes, ISEScan and TnFinder.